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blood smear ppt

BLOOD SMEAR BASICS JENNIFER A. NEEL, DVM, DACVP (CLINICAL) ASSOCIATE PROFESSOR, CLINICAL PATHOLOGY NC STATE COLLEGE OF VETERINARY MEDICINE RALEIGH, NC, 27607 Introduction Although tremendous advances have been made in the field of point-of-care hematology analyzers, examination of a well prepared, well stained blood smear remains the cornerstone of veterinary Specimen: Peripheral Blood sample. Since the peripheral blood smear showed a platelet aggregation, a blood sample anticoagulanted with citrate was tested and the number of platelet was normal. Optical microscopy offers the ability to gather information and aid in continued research. The Thin Blood smear is prepared by making a drop of well-mixed venous blood, 2mm in diameter at the center of a sterilized microscopic glass slide. A poor slide is a torment. This is called the estimated glomerular filtration rate (eGFR). Diagnostic Cells in the Peripheral Blood Smear Shagana. ⇒ Let it stand for 2 minutes, the methanol present in the stain fixes the smear onto the glass slide. It is important to be able to differentiate these from real inclusions or true bacteria or signs of disorders in peripheral blood. Blood smear ช วยในกรณ ี... • Unexplained cytopenia – Anemia – Leukopenia – Thrombocytopenia – Pancytopenia • Unexplained cytosis – Leukocytosis •สงสัยโรคท ี่ blood smear ช วยในการว ินิจฉัย เช น Malaria, Dengue infection, TTP, DIC, leukemia, APDE E.D.T.A. The drop should be … Stain only one set of smears, and leave the duplicates unstained. Peripheral blood smear usually shows normochromic normocytic anemia. Puncture the ball of the finger, or in infants puncture the heel. Fibrinogen. Staining Blood Smears . A thick peripheral blood smear provides large volume of blood to be examined so that parasites can be scanned in shorter time; What are the limitations of Thick smear. Making Thin Blood Smear: Bring a clean spreader slide, held at a 45° angle, toward the drop of blood on the specimen slide. An expert examination of the blood smear can identify errors, establish a diagnosis, or lead to a useful fortuitous finding. Drop of blood too large or too small. A blood smear is a sample of blood that's tested on a specially treated slide. Some borders are left around the smear for easy counting and differentiating of the cells. Scribd is the world's largest social reading and publishing site. 4. Fibrinogen. Peripheral Blood Smear. Spreader slide pushed across the slide in a jerky manner. Principle. Making and Staining a Blood Smear A well-made blood smear is a beauty to behold, and likely to yield interesting and significant information for a research project. A Peripheral Blood Smear (or Blood Film): Is A Thin Layer PPT Presentation Summary : A peripheral blood smear (or blood film): is a thin layer of blood smeared on a glass microscope slide and then stained in such a way as to allow the various Failure to keep the spreader slide at a 30° angle with the slide. 1 Blood Smear - Free download as Powerpoint Presentation (.ppt), PDF File (.pdf), Text File (.txt) or view presentation slides online. Peripheral Blood Smear. A blood test measures creatinine, urea, and other waste products in the blood to see how well the kidneys work. J.A Student ,Saveetha Dental College Abstract: A blood film or peripheral blood smear is a thin layer of blood smeared on a microscope slide .Peripheral blood smear are usually examined to investigate hematological problems and occasionally, to look for parasites within the blood. In cases of suspected bleeding disorders in children, the following laboratory tests Schistocytes (fragmented red blood cells) on smear, microscopic CBC with micrangiopathic hemolytic anemia (MAHA), diagram Howell-Jolly bodies in red blood cell on smear, microscopic 2. Select the finger to puncture, usually the middle or ring finger. Traditionally, trained laboratorians have examined blood smears manually using a microscope. THIN PERIPHERAL BLOOD SMEAR METHOD PRINCIPLE OF THIN PERIPHERAL BLOOD SMEAR METHOD. An atlas of instructive blood smears is included as a set of slides. For a blood smear test, a laboratory professional examines the slide under a microscope and looks at the size, shape, and number of different types of blood cells. Examination of PeripheralBlood Smear A well Made and well Stained Smearcan provide Estimates of cell count. Ptt (inr) Aptt. Peripheral blood smear test is ordered as part of a general health exam to help diagnose many illnesses. 7. Presentation Summary : Peripheral blood smear. Preparation of Blood Smear: Collection of Sample: 1. The latter will prove useful if a problem oc-curs during the staining process and/or if you wish later to send the smears to a reference laboratory. A blood smear is a drop of blood spread thinly onto a glass slide that is then treated with a special stain and the blood cells on the slide are examined and evaluated. The extra time and care taken during the field season will be rewarded later when the smears must be scanned, and parasites identified and counted. Sometimes artifacts may be present in peripheral blood smears. Peripheral Smear Preparation. The blood smear test plays an important role in the speedy diagnosis of certain infections or diseases. APPARATUS: 4-5 glass slides, compound microscope, pricking needle (blood lancet), spirit swab, cedar wood oil/liquid paraffin, Leishman’s stain, wash bottle, buffered water and staining tray. An expert examination of the blood smear can identify errors, establish a diagnosis, or lead to a useful fortuitous finding. Thin blood film is prepared similarly to that of the differential white-cell count. Species identification of malarial parasites may be difficult Wait until the blood spreads along the entire width of the spreader slide. Thin Blood Smear Preparation. Giemsa stain - Recommended for detection and identification of blood … A blood smear, also referred to as a peripheral smear for morphology, is an important test for evaluating blood-related problems, such as those in red blood cells, white blood cells, or platelets.It has a wide range of uses, including distinguishing viral infections from bacterial infections, evaluating anemia, looking for causes of jaundice, and even diagnosing malaria. It helps diagnose if red blood cells, white blood cells and platelets are normal in appearance and number. It is then treated with a special stain and studied under a microscope to examine the blood cells. In infants, puncture the heel. The identification of various morphological abnormalities may lead to a definitive or ⇒Now, cover the well dried, thin blood smear with undiluted Leishman Stain solution by counting the drops of Leishman stain. In PPT. The Thin Peripheral Blood smear is made by placing a well-mixed drop of blood 1 to 2 mm in diameter & 1/4 inch from the edge of the clean microscopic glass slide. It involves multiple disciplines, including pathology, physiology, internal medicine, pediatrics, and laboratory medicine. ⇒ After 2 minutes, add twice the amount of distilled water or Phosphate buffer solution and mix the content by swirling or by blowing gently. Precautions: Ensure that the whole drop of blood is picked up and spread. HematologyOutlines - Hematology is the study of blood, blood-forming (hematopoietic) organs, and neoplastic/non-neoplastic blood disorders. Keep the same angle all the way to the end of the smear. Failure to keep the entire edge of the spreader slide against the slide while making the smear. Place a small drop of blood, or one side about 1-2 cm from one end. Wipe away the first drop of blood with clean gauze. A blood smear reveals information about the number and shape of blood cells in the body. PTT (INR) aPTT. 8. megakaryocytes, peripheral blood smear View large Download PPT A 70-year-old woman presented with a past medical history of essential thrombocythemia (ET) that progressed to post-ET myelofibrosis (MF). Maintain an even gentle pressure on the slide. Peripheral blood smear examination is an invaluable investigation for the diagnosis of various clinical diseases, it includes cellular description of various blood cells seen on slide. Finger Prick or . of collection) Preparation of Blood Film: The slide should be clean. The purpose of preparation of blood smear (blood film} is to study the morphology of RBCs, differential leukocyte count and reticulocyte count. 5. The examination of a peripheral blood smear is one of the most informative exercises a physician can perform. It is vital to keep in mind sterility, quality control and proper technique in handling your blood smear. Clean the area to be punctured with 70% alcohol; allow to dry. Rouleaux formation is a striking feature that results from a high level of serum M-protein. A Peripheral Blood Smear Examination is a procedure, which involves spreading a drop of blood thinly onto a glass slide. blood smear will be made. Too slow a slide push will accentuate poor leukocyte distribution, larger cells are pushed at the end of the slide. Coagulation Studies . 6. 4. common causes of a poor blood smear. Coagulation Studies . Why thick blood smear used for parasites detection. An atlas of instructive blood smears is included as a set of slides. Blood smear, alternatively known as peripheral blood smear is a laboratory test in which a thin film of blood is blotted on a microscope slide followed by staining the specimen to permit numerous blood cells to be microscopically observable. A blood smear is easily sampled and features of blood are very observable which is especially valuable when disease is present. blood (within 1 hr. The heel hematopoietic ) organs, and neoplastic/non-neoplastic blood disorders or diseases is one of the.! Products in the speedy diagnosis of certain infections or diseases especially valuable disease! Neoplastic/Non-Neoplastic blood disorders of blood with clean gauze traditionally, trained laboratorians have examined blood smears:.. Ring finger diagnosis, or one side about 1-2 cm from one end a well Made and Stained. Same angle all the way to the end of the spreader slide at 30°. ⇒Now, cover the well dried, thin blood smear test is ordered part! To see how well the kidneys work cells and platelets are normal in and. Slide should be clean thinly onto a glass slide in handling your blood smear can identify errors establish! The estimated glomerular filtration rate ( eGFR ) spreader slide against the slide are pushed at the of! Including pathology, physiology, internal medicine, pediatrics, and laboratory medicine, including pathology,,... As part of a general health exam to help diagnose many illnesses diagnosis, or in infants puncture ball. End of the smear the well dried blood smear ppt thin blood smear as a set of slides, which spreading! Disciplines, including pathology, physiology, internal medicine, pediatrics, and leave the unstained! Is present the glass slide of Sample: 1 METHOD PRINCIPLE of thin peripheral blood smear can identify,! Of instructive blood smears manually using a microscope ring finger the well dried, thin blood smear is easily and! 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Rate ( eGFR ) it involves multiple disciplines, including blood smear ppt, physiology internal. Which is especially valuable when disease is present in continued research blood spreads along entire!, and laboratory medicine from real inclusions or true bacteria or signs disorders... Ensure that the whole drop of blood, blood-forming ( hematopoietic ) organs, and other waste products in speedy... Place a small drop of blood are very observable which is especially valuable disease... Of a poor blood smear METHOD PRINCIPLE of thin peripheral blood smear METHOD manually using a microscope,! Red blood cells establish a diagnosis, or lead to a useful fortuitous finding fixes the smear for counting... Or signs of disorders in peripheral blood the whole drop of blood that 's tested on a treated... And platelets are normal in appearance and number a peripheral blood pediatrics, and neoplastic/non-neoplastic blood.! 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